human chondrocyte growth medium  (Cell Applications Inc)

Journal: Medicine

Article Title: Exploration of the molecular mechanism of Wufu Yin in the treatment of knee osteoarthritis based on network pharmacology and experimental validation

doi: 10.1097/MD.0000000000040625

Figure Lengend Snippet: Effect of WFY on the cell viability and inflammatory response of chondrocytes. (A) Cell viability of chondrocytes after 24 hours of action of different concentrations of WFY. (B) Effect of WFY on cell viability of IL-1β-induced chondrocytes. (C and D) The effect of WFY on TNF-ɑ and IL-6 secretion by chondrocytes was assayed using ELISA. All data are presented as mean ± SD (n = 3). WFY = Wufu Yin.

Article Snippet: These cells were cultured using complete growth medium formulated for human articular chondrocytes (Catalog No. CP-H096, Procell Technology, Wuhan, China) under standard incubation conditions at 37 o C in a humidified atmosphere containing 5% CO 2 .

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Medicine

Article Title: Exploration of the molecular mechanism of Wufu Yin in the treatment of knee osteoarthritis based on network pharmacology and experimental validation

doi: 10.1097/MD.0000000000040625

Figure Lengend Snippet: Effect of WFY on the PTGS2 and p38 activation of IL-1β-Induced chondrocytes. (A–D) Western blot detection image and semiquantitative analysis of PTGS2, p38, p-p38 protein levels. All data are presented as mean ± SD (n = 3). p-p38 = phosphorylated p38, WFY = Wufu Yin.

Article Snippet: These cells were cultured using complete growth medium formulated for human articular chondrocytes (Catalog No. CP-H096, Procell Technology, Wuhan, China) under standard incubation conditions at 37 o C in a humidified atmosphere containing 5% CO 2 .

Techniques: Activation Assay, Western Blot